FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8634668
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCA58000.019.310458
TAGTACC28350.018.88767841
ATAGTAC28950.018.1335540
ATTAATC57750.017.5757527
TCATTAC67050.017.38234112
GTCCCTA21950.016.7474381
TAATCAT69850.016.6360269
GTCCTAT45500.016.53759451
TACCTCG33400.016.45123316
ATCATTA74300.015.968945511
GGTCCTA34550.015.70087450
CATGCTA49900.015.01192768
ATTACCT50850.014.86673414
TATTAAT60550.014.7999956
CTATTAA48300.014.7126965
AATAGTA40850.014.65022439
CCTATTA39750.014.61884
ATGCTAA50800.014.53958569
GTACCAA33800.014.49611343
TGTGGTG63300.014.2695291
Produced by FastQC (version 0.11.5)