FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9201306
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA17300.016.998431
GTCCTAT33550.016.27329651
TAGTACC20800.015.31163341
ATTAATC42800.014.9650827
CCTATTA32950.014.4483754
GGTGGTG154400.014.4433441
CATGCTA38250.014.36715268
GGTCCGT46200.014.0175575
TTAATCA49650.013.8872548
TCATTAC53550.013.79059312
TGTGGTG71000.013.7076211
TCCTATC39000.013.371037552
GGCCCGT32500.013.2494361
CAAACCG38150.013.211266510
ATAGTAC24650.013.20412140
GGACCCA38450.013.10719147
ATTACCT42250.013.0882314
CGTGTTA29500.013.0510329
TCCGTGT49700.013.0282827
ATGCTAA41400.013.02063969
Produced by FastQC (version 0.11.5)