FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9937593
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA19650.017.9907461
GTCCTAT36350.015.02027151
ATTAATC49400.014.9477547
TAGTACC24050.014.70203741
GGTCCGT50800.014.4669195
TTAATCA54750.014.1902258
TCATTAC57100.014.09652812
CCCTAGG23200.014.029263
CGTGTTA32050.013.9766269
ACGGGTC50400.013.95701420
TCCGTGT53800.013.8553587
CATGCTA40900.013.77641268
AGACGGG56550.013.67700718
GCCCTAG24850.013.37928662
GACGGGT53250.013.27587319
CTAGGCT26200.013.22420865
CCTAGGC24900.013.21186864
GTCGGGT48650.013.16422424
AAGACGG57850.013.12767317
GGACCCA42250.013.08956247
Produced by FastQC (version 0.11.5)