FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8413456
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA21200.018.6559931
GTCCTAT38050.018.39608251
TAGTACC28200.017.75223541
TTAATCA55850.017.294898
ATTAATC51000.017.0181927
TACCTCG29300.016.84170216
TCATTAC58250.016.82273312
CCTATTA35900.016.2799824
CATGCTA44950.016.19500468
TAATCAT62550.016.0018659
ATAGTAC31950.015.88976540
CGACTAG18450.015.74404113
ATCATTA64300.015.67521211
ATTACCT45950.014.92811814
GGTCCTA33550.014.91714750
ATGCTAA47800.014.86330969
CTATTAA47000.014.5945315
TCCTATC46450.014.46673952
TGCTAAT47500.014.36765270
CATTACC48400.014.31707513
Produced by FastQC (version 0.11.5)