FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9991550
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCA58600.014.5720928
CGTGTTA29050.014.2156119
GGTCCGT43650.014.111015
TCATTAC61950.013.84065412
TCCGTGT48100.013.3148347
CATGCTA43450.013.04911368
GTCCCTA18300.013.0063991
ATTAATC51150.012.9998297
ATAGTAC27550.012.96230340
TAATCAT67500.012.9099719
TAGTACC25850.012.86533341
ACGGGTC44000.012.72621220
AGACGGG51600.012.47964318
GGCCCGT34500.012.4791571
TGGGGTG60400.012.4015531
ATCATTA72100.012.37754811
CAAGACG52050.012.30451216
GTCCGTG52650.012.23063856
ATGCTAA44750.012.20071169
AAGACGG51350.012.19962917
Produced by FastQC (version 0.11.5)