FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7421478
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGT30400.016.120485
ACGGGTC31300.015.20704720
TAGCCGA29050.014.94015734
TCCGTGT34500.014.7102387
GTCGGGT30150.014.62646624
GACGGGT31700.014.57334119
ACCCCGT29750.014.35225952
CCGACGT30250.014.34748837
GGCCCGT18500.014.1929141
CGTACGT8200.014.084619
AAGACGG36800.014.07532317
CAAGACG37100.013.96150616
CCGTGTT37750.013.8146578
AGACGGG37250.013.71737718
CCCCGTG31350.013.6198653
GTCCGTG36400.013.5594236
TCGGGTG33450.013.39284825
GTACGTA8900.013.37024120
ACGTCGC33300.013.34780540
TGGTCCG38800.013.2619134
Produced by FastQC (version 0.11.5)