FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8276586
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCC32000.024.38970428
CTCACTA29700.023.4501516
AGATTAC34750.022.7617326
TAATGCA56450.022.38639370
GCTAATG58600.021.44375868
ATTAATC84900.021.437347
ATAGACT60300.021.24438938
AATAGAC60450.021.19167537
AAACTCG33200.021.08354255
GATTACC37400.021.05535327
TTAATCA90150.021.0430558
AACTCGT34250.020.94811256
TAGACTT61700.020.7608439
ACTCACT34250.020.74361615
AGTCATG64750.020.05317547
CTATTAT64350.019.8516256
CATTACT70300.019.8146913
ACTAAAG65500.019.71669417
CTAAAGC65600.019.52658318
ACTCGTT37250.019.44928257
Produced by FastQC (version 0.11.5)