FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8340971
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGG22250.017.14640663
TCATTAC61950.017.00486412
TTAATCA66650.016.3312198
CCTAGGC24150.015.94243964
GCCCTAG24700.015.87075962
TAATCAT72550.015.244329
ATCATTA72050.015.05838111
TGTCCTA30600.014.63958345
GACGTAT15150.014.5561386
GGCCCGT25400.014.3339981
CTAGGCT26750.014.26213465
GTTAATC35100.014.1591447
TGTGGTG58800.014.0508431
GTCCCTA15600.013.6890391
AATTGAC35800.013.39403737
AATCATT84050.013.07510210
TGGGGTG51750.012.9208391
CGCCCTA30400.012.89491361
GCCCGTA22100.012.6707692
TACAAGC37250.012.59002116
Produced by FastQC (version 0.11.5)