FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8943690
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAC67400.014.7462912
GACGTAT19050.014.3291866
TTAATCA67700.014.2155458
TGTCCTA31000.013.88857245
CCCTAGG19300.013.78176763
TGTGGTG63700.013.1876251
ATCATTA75950.013.17824911
TAATCAT77100.012.8455169
CCTAGGC21600.012.80028264
CGACGTA20650.012.7105145
GTCCCTA16600.012.4405241
GTTAATC40050.012.4081017
GTGCCGT43700.012.1746621
GTGTACC27850.011.94069533
GGCCCGT29100.011.9079491
ACATAAC23150.011.6426432
GCCCTAG23800.011.61693262
GGGGACA32500.011.5237861
AATCATT87350.011.49842710
GGGGTGT33250.011.369121
Produced by FastQC (version 0.11.5)