FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8855253
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT36600.019.31611651
TAGTACC28100.018.68237141
TTAATCA54050.018.1956488
ATAGTAC29150.018.12948440
CATGCTA44600.017.18740368
ATTAATC46600.016.7483087
TCATTAC59150.016.74503712
GTCCCTA17400.016.4986721
GGTCCTA32750.016.13671750
TAATCAT64050.015.8465879
TACCTCG33600.015.83274716
ATGCTAA46250.015.36367469
TCCTATC46150.015.09144852
ATCATTA67900.015.05107511
TTACCTC39300.014.78300115
AATAGTA38350.014.51038239
GTACCAA35750.014.39091243
TGCTAAT48550.014.3483470
ATTACCT50200.014.15286314
GGTGGTG141800.013.9988151
Produced by FastQC (version 0.11.5)