FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9420295
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC29550.017.88959541
ATAGTAC29900.017.68243840
GTCCTAT41350.017.35178251
GTCCCTA20500.016.7318881
TTAATCA59250.016.3608728
CATGCTA44050.016.20782368
ATTAATC51200.015.9257927
TCATTAC63250.015.71366612
GGTCCTA35100.015.35596450
ATGCTAA47700.014.45392969
TAATCAT69450.014.3107099
TCCTATC49600.014.112679552
GTACCAA37500.014.00223143
TGTGGTG66550.013.9896321
ATCATTA71200.013.95897111
TGCTAAT49800.013.84450170
TACCTCG37250.013.62307716
TACCAAG40950.013.33538744
CCTATTA38150.013.3011294
ATTACCT53950.013.298223514
Produced by FastQC (version 0.11.5)