FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8969513
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT13700.025.54606619
GTACGTA14450.023.73574320
TACGTAG14650.023.17319921
TAGCCGA38550.021.42723334
CGCCCTA37950.021.30462361
CGATCTA17200.019.73730547
GGTCCGT42250.019.5534925
CCTAGGC41600.019.43523864
CAAGACG44700.019.33916
CCGACGT42800.019.29942337
CCCTAGG42950.018.74296663
GCCCTAG44000.018.69342462
GACGGGT52850.018.07849519
CTAGGCT45350.017.90531265
CGTAGCA19550.017.90234623
CGACGTC47600.017.79444738
AAGACGG48550.017.66125117
AAACCGC31200.017.61201311
ACCCCGT46000.017.5755352
CAAACCG31700.017.55513810
Produced by FastQC (version 0.11.5)