FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9796512
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT14400.033.05265419
GTACGTA15300.031.33711820
TACGTAG16250.029.07392521
CGATCTA19650.024.04577647
CGTAGCA22650.021.32241223
CGCCCTA38000.020.26261
TCGTACG23600.020.16782418
TAGCCGA43250.020.0749834
GGTCCGT46850.019.9443785
ACGTAGC26300.018.89548722
CAAGACG49550.018.71682216
GCCCTAG42550.018.3420862
CCGACGT48000.018.30907237
TTTCGTA26300.018.09727116
CCCTAGG43350.018.08459563
AAGACGG52100.017.66648717
CCTAGGC45000.017.65454764
GTCGGGT50400.017.56781224
AAACCGC36000.017.20638311
CAAACCG36500.017.1624410
Produced by FastQC (version 0.11.5)