FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682598
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT15800.023.47909419
TACGTAG16450.022.12573821
GTACGTA17650.020.62154420
CAAACCG40000.019.51060510
CGCCCTA35350.019.20714661
CGATCTA18400.019.02205347
ACCGACG40950.018.97486730
AAACCGC40550.018.90075111
GCCCTAG36350.018.77484162
GGTCCGT46300.018.216285
CCTAGGC37800.018.05473364
CCCTAGG38900.017.6341663
TAGCCGA46800.017.28047834
CGTAGCA21500.017.25422523
ACACCGA46000.016.81453928
GTGTACC46150.016.68680833
ACGAGCT46000.016.66770734
CGGGATT61300.016.55655915
TAGGTAC25600.016.54136311
AACCGCC48000.016.47756612
Produced by FastQC (version 0.11.5)