FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8500536
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGG22800.017.8072763
CCTAGGC22950.017.69088464
GCCCTAG23300.017.42513862
TAGTACC24750.016.96903841
GTCCTAT34100.015.60047751
CTAGGCT26050.015.58581665
GTCCCTA18550.015.2863841
ATAGTAC28500.014.98178740
TACCTCG27800.014.35204616
TTAATCA50750.014.2065138
CATGCTA38650.013.94660368
TACGTAG10400.013.79762321
GTACGTA11450.013.75484520
CGTACGT10500.013.66621819
CGCCCTA28900.013.56412661
CCTATTA30650.013.4764924
TAATCAT56900.013.4707179
GGTCCTA32500.013.46093850
ATTACCT40100.013.44103414
TCCTATC39200.013.39233952
Produced by FastQC (version 0.11.5)