FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8739968
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC21350.016.725441
GCCCTAG21500.015.46465162
GTCCTAT31550.015.19816251
CCCTAGG20550.014.98738363
TTAATCA51300.014.598538
CCTAGGC22600.014.40213964
ATAGTAC26250.014.13824740
TCATTAC53550.013.98522712
CTAGGCT25850.013.67432365
GTCCCTA17850.013.5302431
ATTAATC45450.013.3206357
CCTATTA30650.013.2446434
ATCATTA57600.013.18408611
CATGCTA37200.013.07748968
CGTGTTA24400.013.0516179
TACCTCG26650.012.86905316
TAATCAT59750.012.8268189
GGTTGGG46050.012.5414881
CGCCCTA27100.012.5273761
GGTCCGT38600.012.4206985
Produced by FastQC (version 0.11.5)