FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10600596
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC30500.017.90096341
ATTAATC59750.017.0456247
TTAATCA72500.016.5582318
GTCCTAT44050.016.1287951
TACCTCG33550.016.06504216
TCATTAC75100.015.70535712
ATAGTAC36050.015.53339540
ATCATTA79500.015.36441811
TAATCAT83050.014.8340869
CCTATTA40500.014.6930914
ATTACCT49000.014.21381614
CATTACC56400.014.1484713
AATAGTA41950.014.09958739
GTACCAA37700.013.83236943
CTATTAA52200.013.7468325
TATTAAT66300.013.7272396
TTACCTC46150.013.271502515
CATGCTA56300.013.24262468
AATCATT93650.013.19242910
CCCTAGG25800.013.15891563
Produced by FastQC (version 0.11.5)