FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10483846
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA12250.017.99926820
CGTACGT12900.017.36363619
TACGTAG12550.017.29013421
GGTCCGT62450.016.5913665
TAGTACC28000.016.37433441
GTCCCTA25250.016.360531
CGGGATT87100.015.87206615
GCGAGTG87900.015.60793724
TCGGCCC89800.015.2393098
TCCGTGT68950.015.1775547
CAAGACG69300.014.8478816
CGAGTGC93350.014.80918225
ATTCGGC95300.014.7265819
GGCGAGT95800.014.54011823
CGTGTTA42850.014.5391229
GTCCGTG71550.014.5301376
TTGGTCC69250.014.5069693
TAGCCGA59800.014.45717534
AAGACGG70500.014.4462217
TACCTCG32250.014.43352116
Produced by FastQC (version 0.11.5)