FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8782642
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT42250.019.7978251
TAGTACC31900.019.52857641
ATAGTAC33500.018.8048140
ATTAATC51250.018.7118937
TCATTAC58400.018.39846612
ATTACCT48800.018.14457714
CATGCTA47600.017.86929168
TTAATCA58850.017.5442228
GGTCCTA37400.017.03113250
TACCTCG36550.016.94843516
CATTACC54400.016.79162613
ATGCTAA49100.016.75316469
GTACCAA37300.016.60755343
ATCATTA65550.016.55179411
TCCTATC51150.016.4900752
TACCAAG39500.016.2141944
TGCTAAT52250.016.21303270
CCTATCT53050.015.89956553
CCTATTA38750.015.6282234
AATAGTA46850.015.61278639
Produced by FastQC (version 0.11.5)