FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9434097
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT46100.019.3591851
TAGTACC33500.019.01877241
ATAGTAC34800.018.31131440
ATTAATC56100.017.6538337
CATGCTA49750.017.58767768
TTAATCA61000.017.497888
GGTCCTA39350.017.0768950
TACCTCG38300.016.99578316
AATAGTA46450.016.58295639
TCATTAC66500.016.1561112
ATTACCT56400.016.13322614
CCTATTA41850.015.5537564
ATGCTAA56200.015.44452769
GTCCCTA22750.015.386261
GTACCAA41800.015.15752443
CGCAAAC57000.015.04267726
TCCTATC58400.015.04226552
TAATCAT72200.015.0259629
CTATTAA49850.014.9531355
TGCTAAT56650.014.95083670
Produced by FastQC (version 0.11.5)