FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8636775
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA19750.019.4992581
TAGTACC26350.019.25901441
GTCCTAT40900.017.96977451
ATTAATC46000.017.9562637
TTAATCA52650.017.0177978
ATAGTAC30450.016.89572140
TCATTAC56950.016.71598212
CCTATTA32700.016.6994234
ATTACCT44400.016.23803314
ATGCTAA45950.016.15046569
CATGCTA46800.016.08152868
GGTCCTA33100.015.96588850
AATAGTA41300.015.93141939
CATTACC47300.015.83449913
TGCTAAT46600.015.70065270
GTACCAA33700.015.47392643
TCCTATC47750.015.31879552
TACCTCG33450.015.17132716
TACCAAG35900.014.91561544
TTACCTC43700.014.73628715
Produced by FastQC (version 0.11.5)