FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9192079
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC28150.019.64861141
ATAGTAC30800.018.07408340
GTCCTAT44950.017.67471751
GTCCCTA22200.017.6595821
TTAATCA59250.017.4244568
AATAGTA41400.017.252239
ATTAATC52750.017.116817
TCATTAC64400.016.79192712
CATGCTA49350.016.73704568
CCTATTA38200.016.3073064
GTACCAA33250.016.21273243
ATTACCT51000.016.0573414
CATTACC53050.015.76668813
ATGCTAA51800.015.54003569
TACCTCG34150.015.47458216
TGCTAAT51900.015.44249170
GGTCCGT43300.015.437295
TTACCTC45800.015.35892815
ATCATTA72900.015.21807411
GGTCCTA35650.015.21689650
Produced by FastQC (version 0.11.5)