FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9263750
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAATC58850.019.0909657
TTAATCA69250.017.992938
TCATTAC77200.016.68353712
ATCATTA81750.016.09773411
TAATCAT81200.016.077989
TATTAAT67200.015.2630156
CCTATTA41450.015.2013774
CTATTAA54100.015.0765055
TCTAACC25700.014.708055526
GCCCTAG20350.014.44721662
AATCATT93300.014.33031410
TAGTACC25450.014.3016741
GTCCTAT39650.014.12278451
CATTACT43800.013.90369913
ATAGTAC27700.013.77171140
CCCTAGG21050.013.634171563
CGACTAG19200.013.48918513
CCTAGGC21550.013.48017264
CTCTAAC28650.013.31563325
ATTACCC28500.013.14017728
Produced by FastQC (version 0.11.5)