FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8710465
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA21350.020.1691421
TTAATCA56100.018.6538648
TCATTAC59200.018.6228812
ATTAATC54650.018.1883647
ATCATTA64750.016.91862511
CCTATTA38700.016.5526644
TAATCAT65900.016.3580239
CATTACC52100.016.05519913
CTATTAA46850.015.9147675
TATTAAT58100.015.9058956
ATAGTAC25050.015.78760540
GTCCTAT43850.015.32417851
TAGTACC25600.015.17508141
TGGGGTG54600.014.8115161
ATTACCT45500.014.69171514
CATGCTA47400.014.62201468
ATGCTAA48050.014.497478569
AATCATT75800.014.45225410
TCCTATC46400.014.02952652
AATAGTA37750.013.99927539
Produced by FastQC (version 0.11.5)