FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9398080
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCA60600.019.8078568
ATTAATC58600.019.4089367
GTCCCTA24500.019.2866861
TCATTAC67900.018.34860812
TAGTACC27550.017.40892841
TAATCAT71050.017.190159
ATCATTA76050.016.8422511
CATTACC56750.016.83525513
ATAGTAC29600.016.79647840
GTCCTAT48500.016.30897151
CCTATTA44700.015.9713234
ATTACCT50400.015.90137914
AATAGTA42350.015.790791539
TATTAAT66550.015.1973556
CATGCTA52450.014.94688968
AATCATT85450.014.825685510
TTACCTC47450.014.60356115
CTATTAA54750.014.1262345
AAATAGT54250.014.06979138
TACCTCG36300.013.97948616
Produced by FastQC (version 0.11.5)