FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8827771
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA17050.016.6289041
TAGTACC20800.016.3256541
GTCCTAT30900.015.63090951
CCTATTA32500.014.2137914
TCATTAC52200.014.14608412
GGTCCTA24900.013.91528550
ATAGTAC25450.013.89432640
TTAATCA52850.013.7070738
ATTAATC51550.013.3059777
ATCATTA60250.012.72063811
GGCCCGT31900.012.5088431
GGTCCGT33000.012.40775
TAATCAT60800.012.2026489
CATGCTA39300.012.20068668
GCCCTAG19250.012.18162662
GGGGACA38100.012.035091
GGTGGTG137650.012.0024191
GCCCGTG44000.011.9306832
GTACCAA29400.011.90659743
CCCTAGG17950.011.89416563
Produced by FastQC (version 0.11.5)