FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11896742
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG14750.014.94783321
GGTCCGT56750.014.8003935
CGTACGT16000.014.65489519
ACGGGTC49150.014.45447220
AAGACGG59550.014.39845517
GTCCCTA26650.014.1856541
CAAGACG61800.014.15732716
CAAACCG46800.013.83413210
TAGCCGA48600.013.83029934
AGACGGG64400.013.74884418
TAGTACC32200.013.69847441
GTCCGTG62350.013.6956066
GACGGGT53850.013.64776219
TCCGTGT62350.013.639427
GTCCTAT47950.013.57630151
TTAATCA77200.013.3730588
CGTGTTA38600.013.3277269
GTACGTA17300.013.14912820
GTCGGGT50150.013.04980624
TCATTAC79600.012.8820312
Produced by FastQC (version 0.11.5)