FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8690740
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC20750.014.84282241
TGGGGTG56250.014.7510411
GGTGGTG125400.014.3224291
CCCTAGG20400.014.23991263
CCTAGGC21050.013.96654864
GGACCCA34000.013.79360447
GGCCCGT25800.013.2985171
CTAGGCT23250.013.24727665
ACCCCCC37600.013.218430538
TTAATCA57550.013.135998
ATAGTAC24800.012.98331440
TCGGCCC60900.012.9306288
CGGGATT61400.012.82540315
GTGCCGT40150.012.8182471
GCCCTAG24950.012.76527762
GCGAGTG62300.012.58409224
GTGTACC30900.012.57314433
TAATCAT62600.012.5237129
GACCCAC35750.012.43303348
ATTACCT47200.012.38315614
Produced by FastQC (version 0.11.5)