FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7611410
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT7700.020.45360419
TAGCGAC13350.017.30348212
CAAACCG26550.016.47866610
CGCCCTA19550.016.47058761
CGATCTA9900.016.26187747
GTACGTA9750.016.15310120
TACGTAG10000.016.09946821
ACCGACG28800.015.67656630
AAACCGC27750.015.51361411
CCTAGGC22150.015.16939864
AGGGTAC29950.014.95796856
TAGCCGA30500.014.80337434
AGTCGTA16400.014.72589910
GCCCTAG22200.014.66216162
CGGGATT57100.014.64906315
GTGCCGT21950.014.5137781
AGACGCA22250.014.47124439
CCTCGTA21500.014.1649370
ATACCCC19350.014.10871165
TCGGCCC60600.013.9194158
Produced by FastQC (version 0.11.5)