FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8359258
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT6750.023.33127419
AAACCGC31300.019.23153111
CAAACCG32100.019.1883410
TAGCCGA36200.018.95461534
GTACGTA8400.018.74834620
CAAGACG40400.018.27809116
GGTCCGT39650.017.652715
ACCGACG35650.017.5734530
CGCCCTA22600.017.499461
CCTCGTA25150.017.39492470
CCGACGT39850.016.8684137
ACGTCGC39800.016.80143240
AGACGCA25950.016.45976839
TACGTAG9150.016.44663621
CCCTCGT28500.016.3328769
AACCGCC37600.016.10230312
CGACGTC42150.016.03101338
AAGACGG44400.015.84319817
GTCGGGT42950.015.72601524
ACGGGTC46550.015.71289920
Produced by FastQC (version 0.11.5)