FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8872996
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCA51850.018.4279678
GTCCTAT38200.018.14066151
ATAGTAC26300.018.0980640
TCATTAC56100.017.96762712
TAGTACC26950.017.92128641
ATTAATC46850.017.7796657
CATGCTA42900.017.70517568
CCTATTA35700.016.669034
ATGCTAA43700.016.42007469
ATCATTA62400.016.32194511
GTCCCTA19300.016.143461
TGCTAAT43000.015.8748870
TAATCAT61800.015.8574419
TCCTATC44350.015.54627552
AATAGTA39250.015.24773339
GGTTGGG43300.014.8762621
CTATTAA44550.014.8506745
GGTCCTA29950.014.72397250
TATTAAT53950.014.4043476
CATTACC50300.014.19460413
Produced by FastQC (version 0.11.5)