FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9697152
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCA54300.016.434788
GTCCTAT37200.016.18188951
TCATTAC60700.015.16326912
ATTAATC50900.015.0573977
ATAGTAC27650.014.81502640
TAGTACC27350.014.71983441
CATGCTA43550.014.70678868
TAATCAT64200.014.5545839
GTCCCTA22000.014.4784761
GGTTGGG47200.014.3126321
ATCATTA67350.013.9777811
GGTCCTA30750.013.31639650
TCCTATC46150.013.19542752
ATGCTAA47000.013.0315269
CCTATTA38600.012.5117364
TGCTAAT47950.012.48124570
AAAATAG59350.011.91626337
TATTAAT58750.011.9137346
GACTAGA28350.011.85082114
AATAGTA43250.011.81893839
Produced by FastQC (version 0.11.5)