FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11058104
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAAC52100.021.49638218
ATTAATC58850.020.5181167
TTAATCA66150.020.423048
TAGTACC36900.019.6333841
ATAGTAC38350.019.34726740
AATAGGA57500.019.05160516
CTGGGGG94000.018.9166373
TGGGGGG91850.018.7497714
TCATTAC77800.018.26448212
GGAATAG59300.017.9422914
GAATAGG61150.017.57109815
CCTGGGG85450.017.4912932
GTCCTAT54050.017.48305151
AATAGTA49450.017.48162539
ATCATTA83000.017.41537711
TAATCAT78900.017.2558179
AGGAACG65750.017.24655519
GTCCCTA27900.017.1898291
ATAGGAA65650.017.05958217
CATGCTA57500.016.740968
Produced by FastQC (version 0.11.5)