FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12112947
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC37600.019.92442941
ATTAATC64950.019.6130857
ATAGTAC38300.019.56205240
TTAATCA73750.019.313288
TCATTAC82500.017.9861912
TAGGAAC41350.017.77378818
GTCCCTA27600.017.7554741
GTCCTAT57300.017.46897151
ATTACCT61150.017.11233114
AATAGTA54100.016.9553639
ATCATTA88950.016.9178911
CCTATTA48900.016.6038274
TAATCAT88500.016.4502969
GGTCCTA46050.016.18847550
CTATTAA56750.015.8487975
CATGCTA63650.015.72606268
TATTAAT71000.015.6746496
AAATAGT61100.015.64315838
AATAGGA47250.015.62848516
GTACCAA45000.015.24678943
Produced by FastQC (version 0.11.5)