FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9472749
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT37750.018.17175151
ATTAATC53250.016.627137
TTAATCA60450.016.267718
TCATTAC63550.015.69468312
GTCCCTA21050.015.2980811
TAGTACC25500.014.82693341
GGTCCTA26750.014.39217950
CATGCTA48250.014.071979568
TCCTATC46950.013.94002952
ATCATTA72950.013.86408511
TAATCAT74000.013.6673669
GGTTGGG43050.013.6595791
ATAGTAC27650.013.54862840
CTATTAA47200.013.3459415
AATAGTA43750.013.20421539
ATTACCT48300.013.18732514
TATTAAT61700.013.1589586
CCTATTA39250.013.1067914
ATGCTAA51750.012.98492269
CATTACC52350.012.36746313
Produced by FastQC (version 0.11.5)