FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11706344
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351600.3003499640878484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG19350.022.07686230
TCCCTCG29550.021.664074
TCGGCCC37750.021.2189648
CGGGATT38650.020.72512415
TCGGCGA39500.020.45817421
TTCGGCG40150.020.21332620
ATTCGGC41550.019.95311719
GGATTCG41650.019.82037517
CGAGTGC41600.019.43389725
GCGAGTG41800.019.41982724
CCGGGAT43550.018.95532814
GGCGAGT42550.018.91088323
TCGTACG9600.018.58259813
AAACCGC24300.018.28105411
CGTACGA9700.018.03041614
GATTCGG44950.017.97701518
CGCGGAC24050.017.90721556
CGGCGAG45600.017.87533622
TCGCCGG20850.017.79023648
AACCGCC26200.017.48950412
Produced by FastQC (version 0.11.5)