FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10928472
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336960.3083322169833075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG18500.024.0265930
AAACCGC22400.019.84384311
TCGGCCC33700.019.6291738
CAAACCG23550.019.46930510
CGGGATT34350.019.46151715
CGCGGAC22150.019.2774856
TCGGCGA35200.019.19016621
CGACGAG23000.019.17365332
AACCGCC24350.019.11730812
CCACGCG24150.018.6955751
TTCGGCG35100.018.64655520
TCCCTCG27350.018.1727644
TCGAACC24800.017.92322319
GCGAGTG36550.017.90665224
CGGCGAC24750.017.81826670
GGCGAGT36850.017.66589423
GGATTCG38150.017.52301817
ATTCGGC37600.017.31367319
CGAGTGC38750.017.25129925
GACGAGC26000.017.23053633
Produced by FastQC (version 0.11.5)