FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9342106
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCGA27200.026.753121
TTCGGCG27900.026.33153220
TCGGCCC27550.026.2832818
TCCCTCG25400.025.3421134
CGGAACG8300.024.45429246
GCGAGTG30100.024.41232524
CGGGATT29800.024.06416115
AACGGCG9300.023.70472349
GGCGAGT30650.023.62987123
TCGTTTG31650.023.54214317
CGAGTGC31200.023.44475625
ATTCGGC31700.022.95423319
CGGCGAG31800.022.77411322
TACCCGC9950.021.79713610
GGATTCG33550.021.37477117
CTCGTTT36700.020.39801216
GATTCGG35900.020.36633518
GTTCTCG37800.019.80399513
CCGGGAT36750.019.60833514
TTCGCGA12000.019.54899256
Produced by FastQC (version 0.11.5)