FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8713199
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACG8350.025.14930546
TCGTTTG32350.024.45093217
TCCCTCG23400.024.082894
TCGGCCC25950.023.7382648
AATCGCG9600.022.9685250
TACCCGC8400.022.91656910
GCGTACG5050.022.87092642
TCGGCGA26850.022.81142821
CGTACGG5250.022.6663143
TTCGGCG27700.021.73238620
GTTCTCG36750.021.71431713
CCGACCG9350.021.7115162
AACGGCG8900.021.62899649
CTCGTTT37600.021.5025716
CGGGATT27900.021.4515215
GCGAGTG29300.021.02404224
ATTCGGC29950.020.21659519
GGCGAGT30200.020.16559223
CGAGTGC30900.019.82203725
CGCCCTA18550.019.81157961
Produced by FastQC (version 0.11.5)