FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8584402
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192830.2246283433604344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCCC25750.018.8811518
ACCGACG15750.018.23162530
TCCCTCG20200.017.4903624
CGGGATT28000.017.48883615
TCGTACG7600.017.48863212
TCGGCGA28350.017.39822821
CCGGGAT29600.016.897814
TTCGGCG29300.016.83343520
GCGAGTG29150.016.6851124
GGCGAGT30500.015.9447323
GGATTCG31950.015.76467717
CGGCGAG31650.015.69572622
CGAGTGC30600.015.66924625
ATTCGGC30900.015.62209719
AAACCGC18700.015.52475911
CGCGGAC18400.015.41438956
CGTACGA9100.015.37462113
CAAACCG19400.015.32517910
GATTCGG33100.015.11190318
TCGAACC19600.014.99139119
Produced by FastQC (version 0.11.5)