FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10378876
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA10000.023.10941756
TACCCGC8650.021.83629410
ACCGACG11900.020.30327230
TCGGCCC24950.019.2066358
TCGCGAT11500.019.18580257
TCGTACG6650.018.93564213
TCGCCGG13700.018.90114848
ATTGCCG16800.018.7644362
CGCGGCA14100.018.62586832
ACCCGCG14200.018.48533825
CGTACGG4000.018.375443
TCCCTCG23250.018.205274
ATCGCGC9300.018.05994851
CCGTCGC14350.018.04708745
CGACGAG13700.017.8917132
AGTCCGC15850.017.8754527
CGGAACG6100.017.78470246
GCGAGTG27000.017.6244924
TCGGCGA26650.017.58930221
CGACCCG15150.017.3201823
Produced by FastQC (version 0.11.5)