FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9663730
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG10600.024.43358430
TCGGCCC22150.022.279688
TCGTACG5950.021.17614412
CGTACGA6100.020.6553113
CGGCGAC13300.020.52706170
TCGGCGA23900.020.35512421
TCCCTCG22050.019.8420934
CGACGAG12800.019.41386432
ATTGCCG14950.018.96340862
TACCCGC9550.018.69090710
TTCGGCG26000.018.44192720
AACGGCG7600.018.42114849
CGAGTGC25950.018.34268425
CGCGGAC13950.018.3153256
GCGAGTG26150.018.20239424
CGGAACG7200.017.98620646
CGGGATT26600.017.89455215
GGCGAGT27400.017.88293523
TCGTTTG21750.017.21803717
GACGAGC14700.017.14276933
Produced by FastQC (version 0.11.5)