FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10739198
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127760.11896605314475066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG16450.021.07271630
CGCGGAC19800.018.39082156
TCGTACG8900.017.68661912
CGTACGA9450.017.02740113
CAAACCG21800.016.5274210
CGGCGAC21850.016.18229770
AAACCGC21650.016.15721311
CGACGAG21300.015.94598832
TCGAACC21650.015.67329919
AACCGCC23250.015.49660712
CCACGCG23150.015.26567851
ACACCGA23350.014.84379328
GACGAGC23900.014.65080233
CGAACCC25600.014.21163420
TGCCGAC17750.014.20588764
CGACCCG17050.014.15917823
ACGAGCT25500.014.14348234
GACACCG26250.014.14278960
CCCGTCG16100.014.12981144
CACCGCG27900.014.06003962
Produced by FastQC (version 0.11.5)