FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12332656
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG27150.021.6689330
CGCGGCA38500.019.8290832
ACCCGCG40550.019.6776125
CGACCCG39300.019.40668723
CAAACCG34450.018.37722610
CGCGGAC32300.018.3209856
TCGGCCC54300.018.1008458
CGGGATT54250.018.0527615
CCGTCGC39400.018.03039645
TCGCCGG40150.017.77916348
CCCCGAC42800.017.73520
AAACCGC33450.017.67174511
CCCGTCG40750.017.6055444
CAGTCCG43350.017.5909676
AGTCCGC44400.017.3313377
GCGAGTG57000.017.12640224
ACACCGA35750.017.04135928
AACCGCC35100.017.04011512
GACCCGC45400.016.95522724
TCGAACC36000.016.51809519
Produced by FastQC (version 0.11.5)