FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12161176
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153010.12581842413924443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTG49750.028.12449517
CGGAACG11100.028.05808846
CGACCGT12250.026.2724120
TCCCTCG34400.025.7284624
TCGGCCC42050.025.0390788
AACGGCG12600.024.715449
GTTCTCG56550.024.68063513
TCGGCGA42850.024.32950421
TTCGGCG43500.024.12571520
CGGGATT43950.023.79733715
CTCGTTT61100.023.3580616
GCGAGTG45500.022.76285624
GGCGAGT46500.022.57223
CGAGTGC46500.022.27827825
TTCGCGA22400.022.19534556
CGCGGCA20700.022.16069232
TCGATCG13450.022.1080386
CGGCGAG47950.022.0340922
TGCGGCG59950.022.01570755
AATCGCG7650.021.49645250
Produced by FastQC (version 0.11.5)