FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10022792
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259120.2585307566993309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTCG35250.020.3445744
TCGGCCC44200.020.1813458
ACCCGCG24000.020.12381425
CGGGATT45250.019.86815615
TCGCCGG23000.019.0179648
TTCGGCG48200.018.7262320
TCGGCGA48800.018.4964521
CGCGGCA24300.018.44479232
CCGGGAT50300.018.15144514
GCGAGTG50200.018.12307424
CGAGTGC52600.017.36589425
GGCGAGT52600.017.16200423
CGACCCG27500.017.04928423
GGATTCG53300.016.99911917
CCGTCGC24350.016.9593945
AGTCCGC27850.016.9566767
ATTCGGC52650.016.94405619
CCCGTCG25050.016.90527344
CGGCGAG55500.016.45323222
CAGTCCG27650.016.3210626
Produced by FastQC (version 0.11.5)