FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10713308
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTCG28450.022.2570254
TCGGCCC35600.021.9116468
TCGGCGA35650.021.58836621
CGGGATT36100.021.1238715
TTCGCGA19300.020.86288556
TTCGGCG36900.020.66715420
TCGCGAT19600.020.36881657
CGCGGCA20950.019.72334132
GCGAGTG38800.019.65914224
CGGCGAG38800.019.5655822
CGAGTGC39800.019.2568825
GGCGAGT39650.019.2356323
TCGTTTG34100.019.18313217
ATTCGGC41150.019.12763219
ACCCGCG22500.018.82035325
CGCGATG22200.018.29971558
CGTCAAC24050.018.19906235
CAGTCCG24150.017.9627726
ATCGCGT22750.017.85480331
CGACCCG23150.017.68186223
Produced by FastQC (version 0.11.5)