FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9968099
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCCC31000.021.7907078
TCCCTCG28500.021.369874
TCGTTTG31000.020.66082417
CGGGATT33500.020.268315
TCGGCGA33650.020.17805321
CGCGGCA19800.019.79763232
AATCGCG7500.019.59965550
TTCGGCG35800.018.86847920
GCGAGTG36500.018.79466224
TTCGCGA18850.018.7530856
CGGCGAG37550.018.3622322
GGCGAGT37450.018.31780223
CGAGTGC37100.018.30202525
TCGCGAT19350.018.26850557
ACCCGCG21600.018.14828325
TACCCGC12350.018.13769710
ATTCGGC37700.018.01038419
TCGCCGG19350.017.90675248
ACCGACG7050.017.87220630
CGCCCTA22750.017.84628961
Produced by FastQC (version 0.11.5)