FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11346123
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126340.11135081119779858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG11450.017.43216730
CGACCCG21600.016.84521923
TCCCTCG26700.016.5080244
TCGGCCC35700.016.4603318
ACCCGCG22950.016.3173425
TTCGCGA15800.016.17728456
GTTCGCA29200.015.81241516
CGCGGCA24150.015.80509632
TCGGCGA37350.015.73448721
CAGTCCG23650.015.6785796
CGGGATT37250.015.58760415
TCGCCGG21800.015.56973648
CCCGTCG23350.014.98808444
CCGTCGC22200.014.97574145
ATTCGGC39150.014.65326919
TTCGGCG40050.014.49872820
ATCGCGT17900.014.47646731
CCGGGAT40700.014.26635714
GCGAGTG41000.014.1665924
ATTGCCG18700.014.04794562
Produced by FastQC (version 0.11.5)