FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10569732
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123090.11645517596851085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCG21500.020.34855725
CGCGGCA22900.018.18743532
CGACCCG23700.018.16439223
CCGTCGC21950.017.38025345
TTCGCGA13050.017.16459756
TCGCCGG23050.016.85451148
CAGTCCG24750.016.6876666
GTTCGCA27550.016.26127216
ACCGACG11900.016.17624930
TCGCGAT13650.016.15385257
CCCGTCG23300.016.07281544
TCGGCCC32250.015.9537218
GCGGCAC27150.015.46932633
AGTCCGC27350.015.3565697
GCGTACG5700.015.35073942
TCCCTCG29000.015.2075514
ATCGCGT15050.014.88351531
CGACGTC12400.014.67728638
GACCCGC28900.014.65384724
GTGTTCG31150.014.49438814
Produced by FastQC (version 0.11.5)